Inhibition of calcium channel activation in GH3 cells by static magnetic fields

Biochim Biophys Acta. 1996 Jun 13;1282(1):149-55. doi: 10.1016/0005-2736(96)00053-3.

Abstract

Voltage-activated calcium channel function was examined in cultured GH3 cells using the whole-cell patch clamp technique. Exposure to a 120 mT static magnetic field resulted in a slight reduction in the peak calcium current amplitude and shift in the current-voltage relationship. The most significant change was a slowing of the channel activation rate without any change in the inactivation rate. All changes in channel function were reversible, with return to pre-exposure values within 3 min after the field was turned off. These alterations in channel function were temperature-dependent. The present findings are consistent with a functional disruption of the intramembranous portion of the calcium channel by a magnetically induced membrane deformation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Calcium Channels / physiology*
  • Cell Line
  • Egtazic Acid / pharmacology
  • Electric Conductivity
  • Magnetics*
  • Membrane Potentials
  • Patch-Clamp Techniques
  • Pituitary Gland
  • Tetrodotoxin / pharmacology

Substances

  • Calcium Channels
  • Tetrodotoxin
  • Egtazic Acid