Molecular cloning, expression and function of the murine CB2 peripheral cannabinoid receptor

Biochim Biophys Acta. 1996 Jun 7;1307(2):132-6. doi: 10.1016/0167-4781(96)00047-4.


We have cloned the peripheral cannabinoid receptor, mCB2, from a mouse splenocyte cDNA library. The 3.7 kb sequence contains an open reading frame encoding a protein of 347 residues sharing 82% overall identity with the only other known peripheral receptor, human CB2 (hCB2) and shorter than hCB2 by 13 amino acids at the carboxyl terminus. Binding experiments with membranes from COS-3 cells transiently expressing mCB2 showed that the synthetic cannabinoid WIN 55212-2 had a 6-fold lower affinity for mCB2 than for hCB2, whereas both receptors showed similar affinities for the agonists CP 55,940, delta(9)-THC and anandamide and almost no affinity for the central receptor- (CB1) specific antagonist SR 141716A. Both hCB2 and mCB2 mediate agonist-stimulated inhibition of forskolin-induced cAMP production in CHO cell lines permanently expressing the receptors. SR 141716A failed to antagonize this activity in either cell line, confirming its specificity for CB1.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cannabinoids / metabolism*
  • Cloning, Molecular
  • DNA, Complementary
  • Humans
  • Mice
  • Molecular Sequence Data
  • Receptors, Cannabinoid
  • Receptors, Drug / genetics*
  • Receptors, Drug / metabolism
  • Sequence Homology, Amino Acid


  • Cannabinoids
  • DNA, Complementary
  • Receptors, Cannabinoid
  • Receptors, Drug

Associated data

  • GENBANK/X86405