Profilin is required for posterior patterning of the Drosophila oocyte

Development. 1996 Jul;122(7):2109-16. doi: 10.1242/dev.122.7.2109.

Abstract

We have investigated the role of the actin cytoskeleton during mid-oogenesis and have found that disrupting the actin cytoskeleton with cytochalasin D induces microtubule bundling and microtubule-based cytoplasmic streaming within the oocyte, similar to that which occurs prematurely in cappuccino and spire mutant oocytes. After examining a number of mutants that affect the actin cytoskeleton, we have found that chickadee, which encodes the actin-binding protein, profilin, shares this phenotype. In addition to the microtubule misregulation, mutants in chickadee resemble cappuccino in that they fail to localize STAUFEN and oskar mRNA to the posterior pole of the developing oocyte. Also, a strong allele of cappuccino has multinucleate nurse cells, similar to those previously described in chickadee. In an independent line of experiments, we have identified profilin as a CAPPUCCINO interactor in a two-hybrid screen for proteins that bind to CAPPUCCINO. This, together with the similarity of mutant phenotypes, suggests that profilin and CAPPUCCINO may interact during development.

MeSH terms

  • Actins / drug effects
  • Actins / metabolism
  • Animals
  • Base Sequence
  • Cell Division / genetics
  • Contractile Proteins*
  • Cytochalasin D / pharmacology
  • Cytoplasmic Streaming / drug effects
  • Cytoplasmic Streaming / physiology
  • Cytoskeleton / drug effects
  • Cytoskeleton / metabolism
  • Drosophila / embryology*
  • Drosophila Proteins*
  • Insect Hormones / genetics
  • Insect Hormones / metabolism
  • Microfilament Proteins / physiology*
  • Microtubules / drug effects
  • Microtubules / genetics
  • Molecular Sequence Data
  • Mutation
  • Oogenesis / physiology*
  • Profilins
  • Proteins / genetics
  • Proteins / metabolism
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism
  • Transcription, Genetic

Substances

  • Actins
  • Contractile Proteins
  • Drosophila Proteins
  • Insect Hormones
  • Microfilament Proteins
  • Profilins
  • Proteins
  • RNA-Binding Proteins
  • capu protein, Drosophila
  • chic protein, Drosophila
  • stau protein, Drosophila
  • Cytochalasin D

Associated data

  • GENBANK/M84529