Selective inhibition of IL-2 gene expression by trichostatin A, a potent inhibitor of mammalian histone deacetylase

J Antibiot (Tokyo). 1996 May;49(5):453-7. doi: 10.7164/antibiotics.49.453.


During screening for inhibitors of T cell activation, we have found that trichostatin A (TSA), known as a potent inhibitor of histone deacetylase, showed selective inhibitory activity against IL-2 gene expression. From luciferase reporter experiments on human leukemic Jurkat T cells, TSA was found to inhibit the expression of the luciferase reporter gene directed by the IL-2 enhancer and promoter with a 50% inhibitory concentration value of 0.073 microM. On the other hand, TSA, at the same concentration, enhanced the expression of the luciferase reporter gene directed by the c-fos enhancer and promoter. The result of RT-PCR experiments also indicates that TSA has selective inhibitory activity against IL-2 gene expression in Jurkat cells. These results suggest that the change in chromatin structure caused by the hyperacetylation of histone might affect the regulation of IL-2 and c-fos gene expression.

MeSH terms

  • Animals
  • Cells, Cultured
  • Enzyme Inhibitors / isolation & purification
  • Enzyme Inhibitors / pharmacology*
  • Gene Expression / drug effects*
  • Genes, fos / drug effects*
  • Genes, fos / genetics
  • Histone Deacetylase Inhibitors
  • Humans
  • Hydroxamic Acids / isolation & purification
  • Hydroxamic Acids / pharmacology*
  • Hypersensitivity, Delayed / drug therapy
  • Interleukin-2 / genetics*
  • Mice
  • Mice, Inbred BALB C
  • Signal Transduction / drug effects


  • Enzyme Inhibitors
  • Histone Deacetylase Inhibitors
  • Hydroxamic Acids
  • Interleukin-2
  • trichostatin A