Stimulation of IgE and IgA production by CD45RA T helper cells in atopic patients

J Immunol. 1996 Jul 1;157(1):441-8.


The role of CD45RA T cells on allergen-dependent lymphocyte functions was analyzed in atopic patients. As compared with age-matched nonatopic controls, atopic patients exhibited a significantly (p < 0.01) increased frequency of CD45RA T cells in peripheral blood. Concentration of serum IgE correlated with increases in this T cell subset. In contrast to nonatopic controls, not only CD45RO but also CD45RA T cells from atopic patients provide help for allergen-stimulated IgE and IgA production, and they act in a synergistic fashion. Transwell coculture experiments revealed that optimal production of IgE and IgA required physical contact of CD45RA T cells with B cells. Freshly prepared and in vitro-activated CD45RO and CD45RA T cells from atopic patients showed an increased expression of CD40 ligand when compared with nonatopic individuals. In addition, CD45RA (and CD45RO) T cells from atopic individuals produced IL-4, IL-5, and IFN-gamma when stimulated with mitogens. Whereas stimulation of normal lymphocytes with tetanus Ag was followed by conversion of the CD45RA to the CD45RO phenotype, T cells from atopic donors did not acquire the CD45RO isoform to the same degree despite T cell activation. In atopic patients, addition of IL-4 to anti-CD3/anti-TCR stimulated CD45RA T cell prevented the shift towards the CD45RO phenotype. These data indicate that a subset of CD45RA T cells plays a unique role as effector T cells regulating IgE and IgA production in atopic patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens / pharmacology
  • B-Lymphocytes / immunology
  • Cell Communication / immunology
  • Cytokines / biosynthesis
  • Humans
  • Hypersensitivity, Immediate / immunology*
  • Immunoglobulin A / biosynthesis*
  • Immunoglobulin A / blood
  • Immunoglobulin E / biosynthesis*
  • Immunoglobulin E / blood
  • Immunophenotyping
  • Leukocyte Common Antigens / analysis*
  • Lymphocyte Activation
  • Lymphocyte Cooperation
  • Lymphocyte Count
  • T-Lymphocytes, Helper-Inducer / classification
  • T-Lymphocytes, Helper-Inducer / immunology
  • T-Lymphocytes, Helper-Inducer / metabolism*


  • Allergens
  • Cytokines
  • Immunoglobulin A
  • Immunoglobulin E
  • Leukocyte Common Antigens