pMPY-ZAP: a reusable polymerase chain reaction-directed gene disruption cassette for Saccharomyces cerevisiae

Yeast. 1996 Feb;12(2):129-34. doi: 10.1002/(sici)1097-0061(199602)12:2<129::aid-yea891>3.0.co;2-o.

Abstract

Gene disruption is an important method for genetic analysis in Saccharomyces cerevisiae. We have designed a polymerase chain reaction-directed gene disruption cassette that allows rapid disruption of genes in S. cerevisiae without previously cloning them. In addition, this cassette allows recycling of URA3, generating gene disruptions without the permanent loss of the ura3 marker. An indefinite number of disruptions can therefore be made in the same strain.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Genetic Markers
  • Molecular Sequence Data
  • Mutagenesis, Insertional / methods*
  • Orotidine-5'-Phosphate Decarboxylase / genetics
  • Polymerase Chain Reaction
  • Saccharomyces cerevisiae / genetics*
  • Selection, Genetic
  • Transformation, Genetic

Substances

  • Genetic Markers
  • Orotidine-5'-Phosphate Decarboxylase