Short report: simultaneous detection by polymerase chain reaction of mosquito species and Plasmodium falciparum infection in Anopheles gambiae sensu lato

M Bouare; D Sangare; M Bagayoko; A Toure; Y T Toure; K D Vernick

doi:10.4269/ajtmh.1996.54.629

Short report: simultaneous detection by polymerase chain reaction of mosquito species and Plasmodium falciparum infection in Anopheles gambiae sensu lato

Am J Trop Med Hyg. 1996 Jun;54(6):629-31. doi: 10.4269/ajtmh.1996.54.629.

Authors

M Bouare¹, D Sangare, M Bagayoko, A Toure, Y T Toure, K D Vernick

Affiliation

¹ Departement d'Epidemiologie des Affectations Parasitaires, Ecole Nationale de Medecine et de Pharmacie, Bamako, Mali.

PMID: 8686783
DOI: 10.4269/ajtmh.1996.54.629

Abstract

Total RNA purified from Anopheles gambiae mosquitoes can be used for detection of both 1) infection by Plasmodium falciparum using a reverse transcriptase-polymerase chain reaction (RT-PCR) assay specific for P. falciparum ribosomal RNA (rRNA) of sporogonic stage parasites, and 2) mosquito species using a PCR assay that distinguishes members of the Anopheles gambiae complex.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anopheles / classification*
Anopheles / parasitology
Insect Vectors / classification*
Insect Vectors / parasitology
Plasmodium falciparum / growth & development
Plasmodium falciparum / isolation & purification*
Polymerase Chain Reaction
RNA, Protozoan / analysis*
RNA, Ribosomal / analysis*
Species Specificity

Substances

RNA, Protozoan
RNA, Ribosomal