The glycosylation of Bowes melanoma tissue plasminogen activator: lectin mapping, reaction with anti-L2/HNK-1 antibodies and the presence of sulphated/glucuronic acid containing glycans

Biochem J. 1996 Jun 1;316 ( Pt 2)(Pt 2):427-37. doi: 10.1042/bj3160427.


The glycosylation of tissue plasminogen activator (t-PA) obtained from the Bowes melanoma cell line was re-examined using methods of serial lectin affinity chromatography coupled with Bio-Gel P-4 gel filtration chromatography and exoglycosidase sequencing. This study clarified an earlier discrepancy in the literature and confirmed that the major complex N-linked glycans on Bowes t-PA that carry sialic acid as their sole charged group are bi-antennary, core fucosylated, with terminal N-acetylgalactosamine residues. We also report the characterization of a series of related and previously unidentified sialylated glycans. Further we show that Bowes t-PA expresses glucuronic acid/sulphate containing N-linked glycans and is recognized by anti-carbohydrate L2/HNK-1 monoclonal antibodies. The presence on Bowes t-PA of glycans associated primarily with the nervous system is consistent with its expression in a cell line of neuroectodermal origin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Blotting, Western
  • CD57 Antigens / immunology
  • Carbohydrate Sequence
  • Chromatography, Affinity
  • Chromatography, Gel
  • Chromatography, Ion Exchange
  • Electrophoresis, Paper
  • Epitopes / chemistry
  • Gas Chromatography-Mass Spectrometry
  • Glucuronates / analysis
  • Glycosylation
  • Lectins / metabolism
  • Melanoma, Experimental / chemistry*
  • Molecular Sequence Data
  • N-Acetylneuraminic Acid
  • Oligosaccharides / analysis
  • Oligosaccharides / chemistry
  • Polysaccharides / analysis*
  • Sialic Acids / metabolism
  • Sulfates / analysis
  • Tissue Plasminogen Activator / chemistry*
  • Tissue Plasminogen Activator / metabolism
  • Tumor Cells, Cultured


  • Antibodies, Monoclonal
  • CD57 Antigens
  • Epitopes
  • Glucuronates
  • Lectins
  • Oligosaccharides
  • Polysaccharides
  • Sialic Acids
  • Sulfates
  • glucuronic acid 3-sulfate
  • Tissue Plasminogen Activator
  • N-Acetylneuraminic Acid