Molecular interaction between TLE1 and the carboxyl-terminal domain of HES-1 containing the WRPW motif

Biochem Biophys Res Commun. 1996 Jun 25;223(3):701-5. doi: 10.1006/bbrc.1996.0959.


Groucho is a protein implicated in Notch signaling and involved in segmentation and neural development in Drosophila. Groucho forms transcription complexes with the basic helix-loop-helix proteins encoded by the hairy/Enhancer of split ("hairy-like") gene family. These interactions are mediated by the carboxyl-terminal WRPW motif of Hairy-like proteins. We are interested in determining whether Groucho and its mammalian homologues, the TLE proteins, perform conserved functions. We show that TLE1 interacts with HES-1, a murine homologue of Drosophila Hairy-like proteins, both in the yeast two-hybrid assay and in an interaction assay based on glutathione S-transferase fusion proteins. These results show that Groucho/TLE proteins and Hairy-like/HES proteins are involved in similar interactions in Drosophila and mammals and further suggest that these proteins perform conserved cellular functions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors
  • Binding Sites
  • DNA-Binding Proteins / metabolism
  • Drosophila
  • Glutathione Transferase
  • Helix-Loop-Helix Motifs
  • Homeodomain Proteins / chemistry*
  • Homeodomain Proteins / metabolism*
  • Humans
  • Molecular Sequence Data
  • Nuclear Proteins / metabolism*
  • Rats
  • Recombinant Fusion Proteins / metabolism
  • Repressor Proteins / metabolism
  • Transcription Factor HES-1


  • Basic Helix-Loop-Helix Transcription Factors
  • DNA-Binding Proteins
  • Hes1 protein, mouse
  • Hes1 protein, rat
  • Homeodomain Proteins
  • Nuclear Proteins
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • TLE1 protein, human
  • Transcription Factor HES-1
  • gro protein, Drosophila
  • HES1 protein, human
  • Glutathione Transferase