Involvement of macrophages in the development of encephalomyocarditis (EMC) virus-induced diabetes in mice

Exp Anim. 1996 Jan;45(1):77-80. doi: 10.1538/expanim.45.77.

Abstract

The role of macrophages in the development of diabetes following infection with encephalomyocarditis (EMC) virus was examined in 3 strains of mice (DBA/2 and BALB/c: susceptible, C57BL/6: resistant). After infection with 100 plaque forming units (PFU)/head of EMC-D (highly diabetogenic variant), the incidence of diabetes at 3 days post infection (DPI) (DBA/2: 7/8, BALB/c: 3/8, C57BL/6: 0/8) was well correlated with the severity of macrophage infiltration with beta cell damage in the pancreatic islets (DBA/2: sever, BALB/c: moderate, C57BL/6: slight). Silica-pretreatment depleted macrophage infiltration in the pancreatic islets and decreased the incidence of diabetes at 7 DPI from 100% to 40% in DBA/2 and from 80% to 0% in BALB/c mice, respectively. These results suggest that macrophages play a critical role in the process of pancreatic beta cell damage in EMC virus infection in mice.

MeSH terms

  • Animals
  • Cardiovirus Infections / pathology
  • Cardiovirus Infections / physiopathology*
  • Diabetes Mellitus, Experimental / pathology
  • Diabetes Mellitus, Experimental / physiopathology*
  • Encephalomyocarditis virus*
  • Islets of Langerhans / drug effects
  • Islets of Langerhans / pathology
  • Macrophages / drug effects
  • Macrophages / pathology
  • Macrophages / physiology*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Silicon Dioxide / pharmacology

Substances

  • Silicon Dioxide