Tomato Prf is a member of the leucine-rich repeat class of plant disease resistance genes and lies embedded within the Pto kinase gene cluster

Cell. 1996 Jul 12;86(1):123-33. doi: 10.1016/s0092-8674(00)80083-5.


In tomato, resistance to Pseudomonas syringae pv. tomato (Pst) strains expressing the avirulence gene avrPto requires the presence of at least two host genes, designated Pto and Prf. Here we report that Prf encodes a protein with leucine-zipper, nucleotide-binding, and leucine-rich repeat motifs, as are found in a number of resistance gene products from other plants. prf mutant alleles (4) were found to carry alterations within the Prf coding sequence. A genomic fragment containing Prf complemented a prf mutant tomato line both for resistance to Pst strains expressing avrPto and for sensitivity to the insecticide Fenthion. Prf resides in the middle of the Pto gene cluster, 24 kb from the Pto gene and 500 bp from the Fen gene.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Bacterial Proteins*
  • Base Sequence
  • Chromosomes, Artificial, Yeast / genetics
  • Cloning, Molecular
  • Cosmids / genetics
  • Genetic Complementation Test
  • Immunity, Innate / genetics
  • Leucine Zippers / genetics*
  • Lycopersicon esculentum / genetics*
  • Molecular Sequence Data
  • Mutation / genetics
  • Plant Diseases / genetics
  • Plant Proteins / genetics*
  • Protein-Serine-Threonine Kinases / genetics*


  • Bacterial Proteins
  • Plant Proteins
  • Prf protein, Lycopersicon esculentum
  • abiC protein, Lactococcus lactis
  • Fen protein, Lycopersicon esculentum
  • Pto protein, Lycopersicon
  • Protein-Serine-Threonine Kinases

Associated data

  • GENBANK/U65391