Nuk Controls Pathfinding of Commissural Axons in the Mammalian Central Nervous System

Cell. 1996 Jul 12;86(1):35-46. doi: 10.1016/s0092-8674(00)80075-6.

Abstract

Eph family receptor tyrosine kinases have been proposed to control axon guidance and fasciculation. To address the biological functions of the Eph family member Nuk, two mutations in the mouse germline have been generated: a protein null allele (Nuk1) and an allele that encodes a Nuk-beta gal fusion receptor lacking the tyrosine kinase and C-terminal domains (Nuk(lacZ)). In Nuk1 homozygous brains, the majority of axons forming the posterior tract of the anterior commissure migrate aberrantly to the floor of the brain, resulting in a failure of cortical neurons to link the two temporal lobes. These results indicate that Nuk, a receptor that binds transmembrane ligands, plays a critical and unique role in the pathfinding of specific axons in the mammalian central nervous system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Animals
  • Axons / chemistry*
  • Gene Expression / physiology
  • Homozygote
  • Immunohistochemistry
  • Ligands
  • Mammals
  • Membrane Proteins / analysis
  • Mice
  • Mutagenesis / physiology
  • Optic Nerve / cytology
  • Optic Nerve / embryology
  • Optic Nerve / enzymology
  • Prosencephalon / chemistry*
  • Prosencephalon / cytology
  • Prosencephalon / embryology
  • Receptor Protein-Tyrosine Kinases / genetics*
  • Receptor Protein-Tyrosine Kinases / metabolism
  • Receptor, EphB2
  • beta-Galactosidase / genetics

Substances

  • Ligands
  • Membrane Proteins
  • Ephb2 protein, mouse
  • Receptor Protein-Tyrosine Kinases
  • Receptor, EphB2
  • beta-Galactosidase