Progressive disruption of acinar cell calcium signaling is an early feature of cerulein-induced pancreatitis in mice

Gastroenterology. 1996 Aug;111(2):481-91. doi: 10.1053/gast.1996.v111.pm8690215.


Background & aims: Disruption of pancreatic exocrine secretion is an important feature of acute pancreatitis. Because cytosolic calcium is a key intracellular messenger controlling pancreatic secretion, this study examined patterns of calcium signaling during the early stages of cerulein-induced pancreatitis.

Methods: Mice were administered hourly intraperitoneal injections of cerulein (50 micrograms/kg), and paired controls were administered saline. Acini were isolated by collagenase from pancreatic tissue harvested after injections 1, 3, 5, and 7 and were loaded with Fura-2. Individual cellular calcium responses to acetylcholine and cholecystokinin were studied using digital imaging.

Results: The proportion of cells maintaining a normal oscillatory calcium response to physiological secretagogue stimulation diminished progressively after increasing cerulein injections. Also, the normal polarized spatial pattern of calcium Increase within individual acinar cells was progressively lost. A sustained response to high-dose stimulation was maintained but with diminishing amplitude. The characteristic calcium response to the Ca(2+)-adenosine triphosphatase inhibitor thapsigargin was maintained, implying that calcium reuptake and extrusion were not impaired.

Conclusions: Progressive disruption of physiological patterns of pancreatic acinar cell calcium signaling, notably in the secretory pole of the cell, is an early feature of pancreatitis induced by cerulein hyperstimulation. These changes may be important in contributing to the disruption of exocrine secretion in acute pancreatitis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholine / pharmacology
  • Acute Disease
  • Animals
  • Calcium / metabolism*
  • Calcium-Transporting ATPases / antagonists & inhibitors
  • Ceruletide
  • Cholecystokinin / pharmacology
  • Endoplasmic Reticulum / drug effects
  • Endoplasmic Reticulum / metabolism
  • Enzyme Inhibitors / pharmacology
  • Male
  • Mice
  • Mice, Inbred Strains
  • Pancreas / drug effects
  • Pancreas / metabolism*
  • Pancreatitis / chemically induced
  • Pancreatitis / metabolism*
  • Signal Transduction*
  • Terpenes / pharmacology
  • Thapsigargin


  • Enzyme Inhibitors
  • Terpenes
  • Thapsigargin
  • Ceruletide
  • Cholecystokinin
  • Calcium-Transporting ATPases
  • Acetylcholine
  • Calcium