Inducible expression of erythroid-specific mouse glycophorin gene is regulated by proximal elements and locus control region-like sequence

J Biochem. 1995 Sep;118(3):593-600. doi: 10.1093/oxfordjournals.jbchem.a124951.

Abstract

Cis-acting elements of the gene for mouse glycophorin, an erythroid-specific membrane glycoprotein, were determined by transient and stable transfection assays using murine erythroleukemia (MEL) cells. Cis-acting elements proximal to the transcription start site of the gene can be separated into the basal promoter (-1 to 191 bp) and the distal element (-133 to -92). The basal promoter contained GGTGG and GATA motifs and the distal element contained GATA-1 and NF-E2 motifs. Deletion analysis of the distal GATA site and its neighboring sequence and DNase-I footprinting/EMSA (electrophoretic mobility shift assay) analysis indicated that induced nuclear factor binding to GATA-1 and its neighboring sequence may be required for expression during MEL cell differentiation induced by dimethyl sulfoxide treatment. The NF-E2 site was also shown to be essential for the promoter activity. An approximately 400 bp far upstream region (-1325 to -948bp) containing the binding motifs for GGGTGG, GATA-1 and NF-E2 showed no enhancing activity when this region was examined by transient transfection assay, but it did show enhancement of the differentiation-specific promoter activity in the stable transfection assay. The far upstream region of mouse glycophorin gene may have a function similar to that of the locus control region (LCR) of human beta-globulin gene cluster.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites
  • Cell Differentiation / physiology
  • DNA-Binding Proteins / metabolism
  • Gene Expression Regulation, Leukemic*
  • Globins / genetics
  • Glycophorins / biosynthesis
  • Glycophorins / genetics*
  • Humans
  • Internal-External Control*
  • Leukemia, Erythroblastic, Acute / genetics*
  • Leukemia, Erythroblastic, Acute / metabolism
  • Leukemia, Erythroblastic, Acute / pathology
  • Mice
  • Molecular Sequence Data
  • Regulatory Sequences, Nucleic Acid
  • Sequence Homology, Nucleic Acid
  • Transfection
  • Tumor Cells, Cultured

Substances

  • DNA-Binding Proteins
  • Glycophorins
  • Globins