Establishment of a new extrahepatic bile duct carcinoma cell line, TFK-1

Tohoku J Exp Med. 1995 Sep;177(1):61-71. doi: 10.1620/tjem.177.61.


A new human extrahepatic bile duct carcinoma cell line (TFK-1) was established from a surgically resected tumor specimen, which was histologically diagnosed as partly papillary adenocarcinoma and partly differentiated tubular adenocarcinoma. The tumor cells cultured in RPMI-1640 medium supplemented with 10% FBS grew as monolayers showing epithelial-like morphology with a population doubling time of 37 hr during exponential growth at passage 40. Chromosome number was distributed in the range of 72 to 76, with a modal number of 73. Tumor markers (CEA, CA19-9, ST-439, DUPAN-2) were negative in culture supernatant and plasma of SCID mice grafted with TFK-1 cells. Though no point mutation at 12 codon of K-ras was detected, expression of c-erb B-2 product and MUC1 antigen was positive. TFK-1 is the third cell line established from extrahepatic bile duct carcinomas in the world literature, and should provide useful information on various aspects of this type of neoplasm.

MeSH terms

  • Adenocarcinoma / metabolism
  • Adenocarcinoma / pathology*
  • Adenocarcinoma / ultrastructure
  • Animals
  • Base Sequence
  • Bile Duct Neoplasms / metabolism
  • Bile Duct Neoplasms / pathology*
  • Bile Duct Neoplasms / ultrastructure
  • Bile Ducts, Extrahepatic / metabolism
  • Bile Ducts, Extrahepatic / pathology*
  • Bile Ducts, Extrahepatic / ultrastructure
  • Biomarkers, Tumor / metabolism
  • Cell Division
  • DNA Mutational Analysis
  • Female
  • Flow Cytometry
  • Genes, ras
  • Humans
  • Immunohistochemistry
  • Karyotyping
  • Male
  • Mice
  • Mice, SCID
  • Middle Aged
  • Molecular Sequence Data
  • Mucin-1 / biosynthesis
  • Transplantation, Heterologous
  • Tumor Cells, Cultured


  • Biomarkers, Tumor
  • Mucin-1