Abstract
The O-polysaccharide moiety of Burkholderia pseudomallei 319a lipopolysaccharide was covalently linked to flagellin protein isolated from the same strain. A glycoconjugate incorporating adipic acid dihydrazide as a spacer molecule elicited high-titer immunoglobulin G responses to both the protein and carbohydrate components of the construct. This immunoglobulin G was capable of protecting diabetic rats from challenge with a heterologous B. pseudomallei strain.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Bacterial / administration & dosage
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Antibodies, Bacterial / biosynthesis
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Burkholderia pseudomallei / chemistry*
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Burkholderia pseudomallei / immunology*
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Diabetes Mellitus, Experimental / complications
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Diabetes Mellitus, Experimental / immunology
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Flagellin / chemistry*
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Flagellin / immunology*
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Glycoconjugates / chemistry
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Glycoconjugates / immunology
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Immunization, Passive
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Immunochemistry
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Immunoconjugates / chemistry*
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Immunoconjugates / immunology*
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Immunoglobulin G / administration & dosage
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Immunoglobulin G / biosynthesis
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Melioidosis / complications
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Melioidosis / immunology
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Melioidosis / prevention & control
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Molecular Structure
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O Antigens / chemistry*
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O Antigens / immunology*
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Rabbits
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Rats
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Rats, Sprague-Dawley
Substances
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Antibodies, Bacterial
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Glycoconjugates
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Immunoconjugates
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Immunoglobulin G
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O Antigens
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Flagellin