Rapid physicochemical detachment, separation and concentration of bacteria from beef surfaces

J Appl Bacteriol. 1996 Jun;80(6):673-81. doi: 10.1111/j.1365-2672.1996.tb03273.x.


A simple, rapid, physicochemical treatment for the removal of viable bacteria from the surface of raw and cooked beef is described. The detachment method was linked to a differential centrifugation step which removed large amounts of particulate food matter and concentrated the detached bacteria. The method increased the numbers of bacteria released from beef surfaces and increased the numbers detected by at least one and a half orders of magnitude, when compared to the traditional 'stomaching' technique. This 1-h separation and concentration method produced cleaner suspensions of bacteria and improved the sensitivity of detection by DEFT and direct plate count.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Collagenases / metabolism
  • Escherichia coli / isolation & purification*
  • Food Microbiology
  • Listeria / isolation & purification*
  • Meat / microbiology*
  • Salmonella enteritidis / isolation & purification*
  • Staphylococcus aureus / isolation & purification*


  • Collagenases