Immunoregulation in onchocerciasis: persons with ocular inflammatory disease produce a Th2-like response to Onchocerca volvulus antigen

J Infect Dis. 1996 Aug;174(2):380-6. doi: 10.1093/infdis/174.2.380.

Abstract

To examine the role of specific cytokines in mediating the clinical manifestations of human onchocercal disease, microfilariae-positive Ghanaian subjects with inflammatory ocular disease were compared with microfilariae-positive subjects without ocular disease. Onchocerca volvulus antigen (OvAg)-stimulated peripheral blood mononuclear cells (PBMC) from subjects with disease produced significantly more interleukin (IL)-10 (with disease = 447.34 vs. without disease = 292.22 pg/mL; P < .01) and IL-5 (with disease = 33.36 vs. without disease = 27.26 pg/mL; P = .02). OvAg-stimulated IL-4 and interferon (IFN)-gamma levels were essentially undetectable in either group. When cytokine mRNA levels were measured by reverse transcriptase-polymerase chain reaction ELISA, persons with disease produced significantly more OvAg-stimulated IL-4, IL-5, and IL-10 mRNA (P = .03, < .01, .05, respectively). No difference in IFN-gamma mRNA production by either group was seen. Addition of neutralizing alpha IL-10 antibody to OvAg-stimulated PBMC increased TFN-gamma production to detectable levels in 20 of 24 persons.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Animals
  • Antigens, Helminth / immunology*
  • Cytokines / analysis
  • Cytokines / genetics
  • Ghana / epidemiology
  • Humans
  • Lymphocyte Activation
  • Middle Aged
  • Onchocerca volvulus / immunology*
  • Onchocerciasis, Ocular / epidemiology
  • Onchocerciasis, Ocular / immunology*
  • RNA, Messenger / analysis
  • Skin / parasitology
  • Th2 Cells / immunology*

Substances

  • Antigens, Helminth
  • Cytokines
  • RNA, Messenger