Characterization of PCP-2, a novel receptor protein tyrosine phosphatase of the MAM domain family

Oncogene. 1996 Jun 20;12(12):2555-62.


DNA sequences encoding a novel member of the receptor protein tyrosine phosphatase (R-PTP) family, termed PCP-2, were identified in a human pancreatic adenocarcinoma cDNA library. Human PCP-2 cDNA predicts a protein of 1430 amino acids with a calculated Mr of 160 kDa. The predicted PCP-2 enzyme consists of a 740 amino acid extracellular region, a single transmembrane domain, and a 666 amino acid intracellular portion. The extracellular sequence contains a MAM (meprin/A5/PTPmu) domain, an immunoglobulin-like domain and four fibronectin type III-like repeats, suggesting that it is a member of the PTPkappa and PTPmu subfamily. The intracellular region contains two tandemly-repeated protein tyrosine phosphatase domains. Northern blot analyses revealed a single transcript of 5.5 kilobases, which is expressed at different levels in many human tissues except spleen and placenta. Upon transfection of PCP-2 cDNA into human embryonic kidney fibroblast 293 cells, a protein with an apparent Mr of 180 000 was detected by immunoblot analysis. This size was reduced to the predicted Mr upon treatment with endoglycosidase F, indicating that PCP-2 is glycosylated and, hence, expressed at the cell surface. A potential role of PCP-2 in cell-cell recognition and adhesion is supported by its co-localization with cell adhesion molecules, such as catenin and E-cadherin, at sites of cell-cell contact.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / genetics
  • Adenocarcinoma / pathology
  • Amino Acid Sequence
  • Antigens, Neoplasm / genetics
  • Antigens, Neoplasm / metabolism
  • Base Sequence
  • Cadherins / metabolism
  • Cell Adhesion
  • Cloning, Molecular
  • Cytoskeletal Proteins / metabolism
  • Humans
  • Molecular Sequence Data
  • Pancreatic Neoplasms / genetics*
  • Pancreatic Neoplasms / pathology
  • Protein Tyrosine Phosphatases / chemistry
  • Protein Tyrosine Phosphatases / genetics*
  • Protein Tyrosine Phosphatases / metabolism*
  • RNA, Messenger / analysis
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Trans-Activators*
  • Tumor Cells, Cultured
  • beta Catenin


  • Antigens, Neoplasm
  • CTNNB1 protein, human
  • Cadherins
  • Cytoskeletal Proteins
  • RNA, Messenger
  • Trans-Activators
  • beta Catenin
  • mammary carcinoma antigen
  • Protein Tyrosine Phosphatases

Associated data

  • GENBANK/X97198