Detection of t(2;5)(p23;q35) translocation by reverse transcriptase polymerase chain reaction and in situ hybridization in CD30-positive primary cutaneous lymphoma and lymphomatoid papulosis

Am J Pathol. 1996 Aug;149(2):483-92.


The t(2;5) generates a chimeric NPM-ALK transcript encoded by the nucleophosmin NPM gene fused to the anaplastic lymphoma kinase gene ALK. Using a reverse transcriptase nested polymerase chain reaction assay we have detected NPM-ALK transcripts within CD30+ primary cutaneous lymphoma and lymphomatoid papulosis (LP). The t(2;5) was identified in 4 out of 9 CD30+ anaplastic lymphomas and in 1 out of 4 CD30+ pleomorphic lymphomas. Moreover, the t(2;5) was detected in 3 out of 10 LPs. All NPM-ALK-positive lymphomas and 1 NPM-ALK-positive LP exhibited a clonal rearrangement of the T cell receptor gamma-chain gene. The t(2;5) was detected in 2 cases of LP without other evidence for a clonal lymphoid population. To identify cells carrying the t(2;5) translocation, we used immunohistochemistry to detect the ALK-encoded p80 protein and in situ hybridization for the specific detection of NPM-ALK transcripts. Both p80 protein and NPM-ALK transcripts were expressed by anaplastic or large CD30+ lymphoma cells with positive NPM-ALK amplification. The presence of t(2;5) in a subset of CD30+ cutaneous lymphoma and LP may indicate a common pathogenesis with a subset of anaplastic nodal lymphoma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Antigens, Neoplasm / analysis
  • Base Sequence
  • Chromosomes, Human, Pair 2 / genetics*
  • Chromosomes, Human, Pair 5 / genetics*
  • DNA, Neoplasm / genetics
  • Gene Rearrangement
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization / methods
  • Keratinocytes / chemistry
  • Ki-1 Antigen / analysis
  • Lymphoma, Large-Cell, Anaplastic / genetics*
  • Lymphoma, Large-Cell, Anaplastic / immunology
  • Lymphoma, Large-Cell, Anaplastic / pathology
  • Lymphomatoid Papulosis / genetics*
  • Lymphomatoid Papulosis / immunology
  • Lymphomatoid Papulosis / pathology
  • Middle Aged
  • Molecular Sequence Data
  • Neoplasm Proteins / analysis
  • Phenotype
  • Polymerase Chain Reaction / methods
  • RNA-Directed DNA Polymerase
  • Recombinant Fusion Proteins / analysis
  • Recombinant Fusion Proteins / genetics
  • Retrospective Studies
  • Translocation, Genetic / genetics*


  • Antigens, Neoplasm
  • DNA, Neoplasm
  • Ki-1 Antigen
  • Neoplasm Proteins
  • Recombinant Fusion Proteins
  • protein p80
  • RNA-Directed DNA Polymerase