Leptin receptors expressed on pancreatic beta-cells

Biochem Biophys Res Commun. 1996 Jul 16;224(2):522-7. doi: 10.1006/bbrc.1996.1059.


Leptin (Ob protein) is a recently isolated hormone produced by adipocytes and is a powerful regulator of satiety centers in the brain. A defect in either leptin production or transmission of the leptin signal in animal models, i.e. ob/ob and db/db mice, respectively, results in a syndrome of obesity and diabetes which closely resembles that which occurs in humans. Leptin release is regulated in part by nutritional status and its expression in adipose tissue is up-regulated by insulin. Since hyperinsulinemia is a primary defect in ob/ob and db/db mice which manifests early in the disease, we postulated that leptin may also regulate insulin release as part of a "adipoinsular' feedback loop. We demonstrate the expression of leptin receptor mRNA in primary rat pancreatic islets and in the insulinoma cell line beta TC-3. Furthermore, we find binding of 125I-leptin to beta TC-3 cells which is significantly displaced by leptin. These findings suggest the possibility that the binding of leptin to its receptor in beta-cells may modulate insulin expression in a negative feedback loop, and thereby may have an anti-obesity effect.

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Northern
  • Brain / metabolism
  • Carrier Proteins / biosynthesis*
  • Carrier Proteins / metabolism
  • Cells, Cultured
  • DNA Primers
  • Diabetes Mellitus / genetics
  • Diabetes Mellitus, Type 2 / genetics
  • Islets of Langerhans / metabolism*
  • Kinetics
  • Leptin
  • Lung / metabolism
  • Male
  • Mice
  • Mice, Inbred Strains
  • Models, Biological
  • Molecular Sequence Data
  • Obesity
  • Polymerase Chain Reaction
  • Proteins / metabolism*
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Cell Surface*
  • Receptors, Leptin


  • Carrier Proteins
  • DNA Primers
  • Leptin
  • Proteins
  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, Leptin
  • leptin receptor, mouse