Detection of autoantibodies to nucleolar transcription factor NOR 90/hUBF in sera of patients with rheumatic diseases, by recombinant autoantigen-based assays

Arthritis Rheum. 1996 Aug;39(8):1313-8. doi: 10.1002/art.1780390808.


Objective: We attempted to clarify the clinical characteristics of Japanese patients with autoantibodies to nucleolar transcription factor NOR 90/hUBF (anti-NOR 90) and to analyze the autoantigenic epitopes recognized by anti-NOR 90.

Methods: Ninety-one patient sera containing anti-nucleolar antibodies (ANoA) by indirect immunofluorescence were collected. Immunoblottings were performed using recombinant fusion proteins expressed from several cloned complementary DNA (cDNA) encoding the NOR 90/hUBF autoantigen.

Results: Anti-NOR 90 were detected in sera from 9 (9.9%) of 91 patients with ANoA. Seven of these patients were diagnosed as having Sjögren's syndrome, 4 had concomitant rheumatoid arthritis, 1 had concomitant systemic sclerosis (SSc), and 2 had SSc alone. All 9 sera were reactive with more than 2 recombinant fusion proteins from cDNA encoding separate regions on the hUBF polypeptide.

Conclusion: The results suggest that while anti-NOR 90 antibodies are rare, they are associated with Sjögren's syndrome in Japanese patients, and that autoimmunity is targeted toward at least 2 separate regions (amino acids 89-310 and 310-633) of the hUBF polypeptide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Autoantibodies / analysis*
  • Autoantibodies / blood
  • Base Sequence
  • Cell Nucleolus / chemistry
  • Cell Nucleolus / immunology*
  • DNA, Complementary / isolation & purification
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / immunology*
  • Epitopes / immunology
  • Female
  • Humans
  • Immunoblotting
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Nucleolus Organizer Region / immunology
  • Pol1 Transcription Initiation Complex Proteins*
  • Prevalence
  • Recombinant Fusion Proteins / immunology
  • Recombinant Proteins / immunology
  • Rheumatic Diseases / epidemiology
  • Rheumatic Diseases / immunology*
  • Transcription Factors / genetics
  • Transcription Factors / immunology*


  • Autoantibodies
  • DNA, Complementary
  • DNA-Binding Proteins
  • Epitopes
  • Pol1 Transcription Initiation Complex Proteins
  • Recombinant Fusion Proteins
  • Recombinant Proteins
  • Transcription Factors
  • transcription factor UBF