Molecular Analysis of the Gene Cluster Involved in Cephalosporin Biosynthesis From Lysobacter Lactamgenus YK90

Appl Microbiol Biotechnol. 1996 Jan;44(5):589-96. doi: 10.1007/BF00172490.


Determination of the nucleotide sequence downstream from the Lysobacter lactamgenus pcbC gene encoding isopenicillin N synthase revealed that five open-reading frames (ORF) including the pcbC gene were tightly linked in the same orientation. Each ORF has the remarkable feature of the protein-coding frame in the DNA sequence with a high G+C content. Expression in Escherichia coli and a comparison of the deduced amino acid sequences with published sequences showed that the gene cluster contained a deacetoxycephalosporin C synthetase (DAOCS) gene (cefE), an ORF having homology with the Cephalosporium acremonium DAOCS/deacetylcephalosporin C synthetase gene (cefEF), an isopenicillin N epimerase gene(cefD), and a beta-lactamase gene. The gene order was pcbC-cefE-ORF3-cefD-beta-lactamase.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cephalosporins / biosynthesis*
  • Genes, Bacterial*
  • Gram-Negative Bacteria / genetics*
  • Molecular Sequence Data
  • Multigene Family*
  • Open Reading Frames
  • Oxidoreductases / genetics*


  • Cephalosporins
  • Oxidoreductases
  • isopenicillin N synthetase

Associated data

  • GENBANK/X56660