Characterization of the cefF Gene of Nocardia Lactamdurans Encoding a 3'-methylcephem Hydroxylase Different From the 7-cephem Hydroxylase

Appl Microbiol Biotechnol. 1996 Jan;44(5):605-9. doi: 10.1007/BF00172492.

Abstract

The cefF gene of Nocardia lactamdurans, encoding a functional 2-oxoglutarate-dependent 3'-methylcephem hydroxylase (deacetoxycephalosporin C hydroxylase) has been found to be closely linked to the pcbC gene in the cephamycin C gene cluster. The open-reading frame is 933 bp long and could encode a protein of M(r) 34,366. Introduction of cefF in the cephamycin-non-producer Streptomyces lividans conferred 3'-methylcephem-hydroxylating activity to the transformants but did not result in hydroxylation at carbon 7 of cephamycin. No 3'-methylcephem hydroxylase activity was observed when the cefF gene was introduced in S. lividans in pIJ699 (a vector containing two transcriptional terminators that prevent read-through expression), which suggests that this gene lacks an independent promoter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cephamycins / biosynthesis*
  • Cloning, Molecular
  • Genes, Bacterial*
  • Methyltransferases / genetics*
  • Mixed Function Oxygenases / genetics*
  • Molecular Sequence Data
  • Multienzyme Complexes / genetics*
  • Nocardia / genetics*
  • Oxygenases / genetics*

Substances

  • Cephamycins
  • Multienzyme Complexes
  • 7-alpha-cephem-methoxylase
  • Mixed Function Oxygenases
  • Oxygenases
  • deacetoxycephalosporin C hydroxylase
  • Methyltransferases

Associated data

  • GENBANK/Z21687