Collagen-binding activity of Prevotella intermedia measured by a microtitre plate adherence assay

Microbiology (Reading). 1996 Jun;142 ( Pt 6):1537-1541. doi: 10.1099/13500872-142-6-1537.


The ability of Prevotella intermedia to bind type I collagen was investigated. A simple method in which bacterial cells were allowed to attach to collagen-coated microtitre plate wells was used to characterize the activity. All strains of P. intermedia tested, as well as those of the closely related species Prevotella nigrescens, showed a capacity to attach to the collagen film. Exponential-phase cultures of P. intermedia demonstrated a greater binding capacity than older cells. Attachment to the collagen film was inhibited by the presence of EDTA, type I and IV collagen, denatured collagen (gelatin), fibrinogen or fibronectin. Pretreatment of bacterial cells with heat (60 degrees C, 30 min) or proteinase K also inhibited the binding. The collagen-binding activity could be solubilized from the bacterial cell surface by incubation with Zwittergent 3-14, a zwitterionic detergent. The collagen-binding capacity of P. intermedia demonstrated in the present study represents a mechanism of colonization allowing these bacteria to attach to a tissue matrix.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / analysis*
  • Bacterial Proteins / isolation & purification
  • Bacterial Proteins / metabolism
  • Bacteriological Techniques / instrumentation
  • Collagen / metabolism*
  • Detergents / pharmacology
  • Fibrinogen / metabolism
  • Fibronectins / metabolism
  • Gelatin / metabolism
  • Hot Temperature
  • Integrins / analysis*
  • Integrins / isolation & purification
  • Integrins / metabolism
  • Molecular Sequence Data
  • Prevotella / drug effects
  • Prevotella / metabolism
  • Prevotella intermedia / drug effects
  • Prevotella intermedia / metabolism*
  • Prevotella intermedia / pathogenicity
  • Quaternary Ammonium Compounds / pharmacology
  • Receptors, Collagen
  • Reproducibility of Results
  • Virulence


  • Bacterial Proteins
  • Detergents
  • Fibronectins
  • Integrins
  • Quaternary Ammonium Compounds
  • Receptors, Collagen
  • zwittergent 3-14
  • Gelatin
  • Fibrinogen
  • Collagen