Vaccination Against Tuberculosis by DNA Injection

Nat Med. 1996 Aug;2(8):888-92. doi: 10.1038/nm0896-888.

Abstract

There are 3 million deaths per annum worldwide due to tuberculosis, and AIDS is compounding the problem. A better vaccine than the live mycobacterium currently in use, bacillus Calmette-Guérin (BCG), is needed. When mice were injected with plasmid DNA encoding a single mycobacterial antigen (65-kDa heat shock protein, hsp65) they made specific cellular and humoral responses to the protein and became immune to subsequent challenge with Mycobacterium tuberculosis. Protection was equivalent to that obtained by vaccinating with live BCG, whereas immunizing with the protein was ineffective. Protection was also obtained with DNA encoding another mycobacterial antigen (36-kDa proline-rich antigen). These results suggest that DNA vaccination might yield improved vaccines to replace BCG.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Bacterial / genetics*
  • BCG Vaccine / administration & dosage
  • BCG Vaccine / genetics
  • BCG Vaccine / immunology*
  • Bacterial Proteins*
  • Base Sequence
  • Cell Line, Transformed
  • Chaperonin 60
  • Chaperonins / genetics*
  • Chlorocebus aethiops
  • DNA, Bacterial / administration & dosage
  • DNA, Bacterial / immunology*
  • Female
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Mycobacterium tuberculosis / genetics
  • Mycobacterium tuberculosis / immunology*
  • Tuberculosis / prevention & control*
  • Vaccination

Substances

  • Antigens, Bacterial
  • BCG Vaccine
  • Bacterial Proteins
  • Chaperonin 60
  • DNA, Bacterial
  • heat-shock protein 65, Mycobacterium
  • Chaperonins