An electrophysiological study of calcium entry during normal human T-lymphocyte activation

FEBS Lett. 1996 Jul 15;390(1):78-80. doi: 10.1016/0014-5793(96)00630-8.


Our aim was to observe whether normal human T-cells respond to mitogenic stimulation with large whole-cell inward currents (composed of identifiable single-channel contributions) when [Ca2+]i is not markedly lowered but instead kept normal or moderately low, as has been reported in human leukaemic Jurkat T-cell line and T-cell clones [Kuno et al. (1986) Nature 323, 269-73; Kuno and Gardner (1987) Nature 326, 301-304; Gardner (1990) Annu. Rev. Immunol. 8, 231-252]. Whole-cell patch recordings showed no such currents in cells otherwise normally responding to depolarisation with the macroscopic IK described in T-lymphocytes and thus deemed viable, in agreement with the notion that Ca2+ influx in normal T-cells enterily depends on depletion of internal stores [Putney (1986) Cell Calcium 7, 1-12; Putney (1990) Cell Calcium 11, 611-624].

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium / metabolism*
  • Calcium Channels / physiology*
  • Evoked Potentials
  • Humans
  • In Vitro Techniques
  • Kinetics
  • Lymphocyte Activation*
  • Membrane Potentials
  • Patch-Clamp Techniques
  • Phytohemagglutinins
  • T-Lymphocytes / immunology
  • T-Lymphocytes / physiology*
  • Time Factors
  • Tumor Cells, Cultured


  • Calcium Channels
  • Phytohemagglutinins
  • Calcium

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