Up-regulation of cyclooxygenase-2 mRNA in the rat spinal cord following peripheral inflammation

FEBS Lett. 1996 Jul 22;390(2):165-9. doi: 10.1016/0014-5793(96)00604-7.


Prostaglandins (PG) have been described as mediators in spinal nociceptive processing after peripheral inflammation. Enzymes essential for PG biosynthesis, cyclooxygenase isozymes COX-1 and COX-2, have not yet been investigated in the spinal cord. In two studies on rats with adjuvant-induced peripheral inflammation levels of mRNA expression of both COX isoforms were analyzed in the lumbar section of the spinal cord using reverse transcription-polymerase chain reaction (RT-PCR) technique. We could show that mRNA of both COX isoforms is expressed constitutively in the spinal cord with COX-2 as the predominant isoform. Six hours after induction of peripheral inflammation, levels of COX-2 mRNA expression were raised significantly in respect to untreated control rats and returned to baseline within 3 days after induction of inflammation. COX-2 might therefore be regarded as the COX isozyme responsible for spinal PG release in nociceptive processing under a peripheral inflammatory stimulus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arthritis, Experimental / enzymology
  • Arthritis, Experimental / genetics
  • Base Sequence
  • Cyclooxygenase 1
  • Cyclooxygenase 2
  • DNA Primers / genetics
  • Inflammation / enzymology*
  • Inflammation / genetics*
  • Isoenzymes / genetics*
  • Kinetics
  • Male
  • Membrane Proteins
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Prostaglandin-Endoperoxide Synthases / genetics*
  • RNA, Messenger / genetics*
  • RNA, Messenger / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Spinal Cord / metabolism*
  • Up-Regulation


  • DNA Primers
  • Isoenzymes
  • Membrane Proteins
  • RNA, Messenger
  • Cyclooxygenase 1
  • Cyclooxygenase 2
  • Prostaglandin-Endoperoxide Synthases
  • Ptgs1 protein, rat