Co-expression of mRNA for type I and type II interleukin-1 receptors and the IL-1 receptor accessory protein correlates to IL-1 responsiveness

FEBS Lett. 1996 Aug 5;391(1-2):104-8. doi: 10.1016/0014-5793(96)00713-2.


Three cell surface molecules participate in Interleukin-1 (IL-1) binding and signal generation, the two distinct types of receptors (type I IL-1R and type II IL-1R) and the IL-1 receptor accessory protein (IL-1RAcP). Low surface expression hampers the detection of all three components on a protein level in most cell types, thus the highly sensitive RT-PCR was used to analyse the mRNA expression in a panel of 18 murine cell types of different hemopoietic lineages and fibroblasts. The transcription of both types of IL-1 receptors was detected in all cell lines tested. In most cell lines the IL-1RAcP was co-expressed with the IL-1 receptors, and only these lines responded to IL-1. However, in three cell lines no mRNA for the IL-1RAcP could be detected, and these cells did not respond to IL-1. These results suggest that the expression of the IL-1RAcP correlates with IL-1 responsiveness and they point to a pivotal role for the IL-1RAcP in IL-1 signal generation.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • B-Lymphocytes
  • Base Sequence
  • Binding Sites
  • Cell Line
  • Cell Nucleus / metabolism
  • DNA Primers
  • Interleukin-1 / pharmacology*
  • Lymphoma, T-Cell
  • Macrophages
  • Mice
  • Molecular Sequence Data
  • NF-kappa B / metabolism
  • Polymerase Chain Reaction
  • Protein Biosynthesis*
  • RNA, Messenger / biosynthesis
  • Receptors, Interleukin-1 / biosynthesis*
  • Receptors, Interleukin-1 Type I
  • Receptors, Interleukin-1 Type II
  • Transcription, Genetic*
  • Tumor Cells, Cultured


  • DNA Primers
  • Interleukin-1
  • NF-kappa B
  • RNA, Messenger
  • Receptors, Interleukin-1
  • Receptors, Interleukin-1 Type I
  • Receptors, Interleukin-1 Type II