Immunohistochemical localisation of the serotonin 5-HT2B receptor in mouse gut, cardiovascular system, and brain

FEBS Lett. 1996 Aug 5;391(1-2):45-51. doi: 10.1016/0014-5793(96)00695-3.

Abstract

We recently reported the cloning of a new member of the serotonin 5-HT2 family, the 5-HT2B receptor. We now report the production and characterisation of a specific antiserum directed against the C-terminal portion of the mouse 5-HT2B receptor. After affinity purification, this polyclonal antibody recognises specifically the mouse 5-HT2B receptor. Immunohistochemical analysis of cryosections from various adult mouse tissues reveals a major 5-HT2B receptor expression in stomach, intestine and pulmonary smooth muscles as well as in myocardium. Furthermore, the antiserum recognises specific areas of the mouse brain, including cerebellar Purkinje cells and their projection areas.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / isolation & purification
  • Antibody Specificity
  • Brain / cytology
  • Brain / metabolism*
  • Cell Line
  • Chlorocebus aethiops
  • Chromatography, Affinity
  • Digestive System / cytology
  • Digestive System / metabolism*
  • Gastric Mucosa / metabolism
  • Immunohistochemistry
  • Intestinal Mucosa / metabolism
  • Intestines / cytology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Myocardium / cytology
  • Myocardium / metabolism*
  • Organ Specificity
  • Rabbits
  • Receptor, Serotonin, 5-HT2B
  • Receptors, Serotonin / analysis*
  • Receptors, Serotonin / biosynthesis
  • Recombinant Proteins / biosynthesis
  • Stomach / cytology
  • Transfection

Substances

  • Antibodies
  • Receptor, Serotonin, 5-HT2B
  • Receptors, Serotonin
  • Recombinant Proteins