Plant virus movement protein dynamics probed with a GFP-protein fusion

Gene. 1996;173(1 Spec No):75-9. doi: 10.1016/0378-1119(95)00678-8.

Abstract

A genetic fusion between the gene encoding green fluorescent protein (GFP) from the jellyfish Aequorea victoria, with that of the Ob-tobamovirus movement protein (MP) resulted in the expression of a fluorescent fusion protein (MP::GFP) that was fully biologically active in mediating the cell-to-cell spread of the Ob-virus. The MP::GFP fusion was used to follow in planta the subcellular trafficking of MP. GFP-tagged MP was transiently expressed and found to be associated with several subcellular compartments and structures including trans-wall structures, presumably plasmodesmata, and filament structures. The MP::GFP fusion can be used to monitor MP association with host proteins and structures, and for the isolation of interacting host components.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cloning, Molecular
  • Green Fluorescent Proteins
  • Kinetics
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism*
  • Plant Leaves / virology
  • Plant Viral Movement Proteins
  • Plants, Toxic
  • Protoplasts / virology
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Scyphozoa
  • Tobacco / virology
  • Tobamovirus / genetics
  • Tobamovirus / metabolism*
  • Viral Proteins / genetics
  • Viral Proteins / metabolism*

Substances

  • Luminescent Proteins
  • Plant Viral Movement Proteins
  • Recombinant Fusion Proteins
  • Viral Proteins
  • Green Fluorescent Proteins