Binding sites for maize nuclear proteins in the subterminal regions of the transposable element Activator

Mol Gen Genet. 1996 Jun 24;251(4):428-35. doi: 10.1007/BF02172371.


Genetic data suggest that transposition of the maize element Activator (Ac) is modulated by host factors. Using gel retardation and DNase I protection assays we identified maize proteins which bind to seven subterminal sites in both ends of Ac. Four DNase I-protected sites contain a GGTAAA sequence, the other three include either GATAAA or GTTAAA. The specificity of the maize protein binding to Ac was verified by using a synthetic fragment containing four GGTAAA motifs as probe and competitor in gel retardation assays. All seven binding sites are located within regions required in cis for transposition. A maize protein binding site with the same sequence has previously been identified in the terminal inverted repeats of the maize Mutator element. Thus, the protein, that recognizes this sequence is a good candidate for a regulatory host factor for Ac transposition.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Binding Sites
  • DNA Footprinting
  • DNA Transposable Elements / genetics*
  • Deoxyribonuclease I / metabolism
  • Molecular Sequence Data
  • Nuclear Proteins / chemistry
  • Nuclear Proteins / metabolism*
  • Substrate Specificity
  • Zea mays / genetics*


  • DNA Transposable Elements
  • Nuclear Proteins
  • Deoxyribonuclease I