The genetics of the repair of 5-azacytidine-mediated DNA damage in the fission yeast Schizosaccharomyces pombe

Mol Gen Genet. 1996 Jun 24;251(4):483-92. doi: 10.1007/BF02172377.


We have recently demonstrated that Schizosaccharomyces pombe cells treated with the nucleoside analogue 5-azacytidine (5-azaC) require previously characterised G2 checkpoint mechanisms for survival. Here we present a survey of known DNA repair mutations which defines those genes required for survival in the presence of 5-azaC. Using a combination of single-mutant and epistasis analyses we find that the excision, mismatch and recombinational repair pathways are all required in some degree for the repair of 5-azaC-mediated DNA damage. There are distinct differences in the epistatic interactions of several of the repair mutations with respect to 5-azaC-mediated DNA damage relative to UV-mediated DNA damage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases*
  • Azacitidine / toxicity*
  • Bacterial Proteins / genetics
  • DNA Damage*
  • DNA Repair*
  • DNA-Binding Proteins / drug effects
  • DNA-Binding Proteins / genetics
  • Drug Resistance, Microbial / genetics
  • Endodeoxyribonucleases*
  • Escherichia coli Proteins*
  • Fungal Proteins / drug effects
  • Fungal Proteins / genetics
  • MutS DNA Mismatch-Binding Protein
  • Mutation
  • Nucleotides / genetics
  • Rad51 Recombinase
  • Radiation Tolerance / genetics
  • Recombination, Genetic
  • Saccharomyces cerevisiae Proteins*
  • Schizosaccharomyces / drug effects
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces pombe Proteins*
  • Sequence Homology, Nucleic Acid
  • Transcription Factors*


  • Bacterial Proteins
  • DNA-Binding Proteins
  • Escherichia coli Proteins
  • Fungal Proteins
  • Nucleotides
  • RAD18 protein, S cerevisiae
  • RHP51 protein, S pombe
  • Rad12 protein, Coprinus cinereus
  • SWI4 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces pombe Proteins
  • Transcription Factors
  • RAD2 protein, S cerevisiae
  • Rad51 Recombinase
  • Endodeoxyribonucleases
  • Adenosine Triphosphatases
  • MutS DNA Mismatch-Binding Protein
  • MutS protein, E coli
  • Azacitidine