Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 198 (3), 371-7

Chilling, Oxidative Stress and Antioxidant Responses in Arabidopsis Thaliana Callus

Affiliations

Chilling, Oxidative Stress and Antioxidant Responses in Arabidopsis Thaliana Callus

D O'Kane et al. Planta.

Abstract

Chilling of Arabidopsis thaliana (L.) Heynh. callus tissue to 4 degrees C led to conditions of oxidative stress, as indicated by increased levels of the products of peroxidative damage to cell membranes. Cellular H2O2 was also observed to increase initially upon chilling but by day 8 cellular levels had declined to below control levels. Although levels of catalase activity remained similar to those in unchilled tissue, activity of ascorbate peroxidase increased between days 4 and 8 of chilling to 4 degrees C. In callus held at 23 degrees C, levels of reduced glutathione remained static whereas they rose in callus held at 4 degrees C. Levels of oxidised glutathione were initially low but increased significantly by day 4 in the chilled callus. At 23 degrees C, however, levels of oxidised glutathione remained low. Between days 1 and 3 at 4 degrees C, levels of glutathione reductase activity increased but by day 8 glutathione reductase activity was similar to that in cells held at 23 degrees C. Exposure of callus to abscisic acid at 23 degrees C also led to increased activities of ascorbate peroxidase and glutathione reductase.

Similar articles

See all similar articles

Cited by 34 articles

See all "Cited by" articles

References

    1. Plant Physiol. 1974 Nov;54(5):696-701 - PubMed
    1. Plant Physiol. 1991 Nov;97(3):1265-7 - PubMed
    1. Plant Cell. 1994 Jan;6(1):65-74 - PubMed
    1. Free Radic Biol Med. 1992 Nov;13(5):557-80 - PubMed
    1. Plant Physiol. 1980 Feb;65(2):407-8 - PubMed

Publication types

LinkOut - more resources

Feedback