Anti-gamma delta T cell antibody blocks the induction and maintenance of oral tolerance to ovalbumin in mice

Immunol Lett. 1995 Dec;48(2):97-102. doi: 10.1016/0165-2478(95)02451-4.


The oral administration of antigens is one of the means of inducing tolerance in adult mammals. In this report, the role of gamma delta T cells in the induction and maintenance of orally-induced tolerance to ovalbumin was investigated. The injection of a monoclonal anti-gamma delta T cell monoclonal antibody blocked the induction of oral tolerance, because the secondary immune responses to ovalbumin in these animals were comparable to the corresponding responses in ovalbumin-immunized control mice. Furthermore, depletion of gamma delta T cells either in vivo or in vitro abolished already established oral-tolerance. The fact that the state of tolerance could be adoptively transferred to naive recipients by CD3+ alpha beta- gamma delta + spleen cells from tolerant mice. These results suggest that systemic oral tolerance is induced and actively maintained by mechanisms involving gamma delta T cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Animals
  • Antibodies, Monoclonal / administration & dosage
  • Antibodies, Monoclonal / pharmacology*
  • CD3 Complex / immunology
  • Cells, Cultured
  • Immune Tolerance*
  • Immunotherapy, Adoptive
  • Lymphocytes / immunology*
  • Mice
  • Mice, Inbred DBA
  • Ovalbumin / administration & dosage*
  • Ovalbumin / immunology*
  • Receptors, Antigen, T-Cell, gamma-delta / immunology*
  • Spleen / cytology
  • Spleen / transplantation


  • Antibodies, Monoclonal
  • CD3 Complex
  • Receptors, Antigen, T-Cell, gamma-delta
  • Ovalbumin