Glutathione S-transferase a major allergen of the house dust mite, Dermatophagoides pteronyssinus

Immunol Lett. 1995 Dec;48(2):103-7. doi: 10.1016/0165-2478(95)02452-2.


Recently, we cloned a new Dermatophagoides pteronyssinus allergen homologous with glutathione S-transferase. IgE radioimmunoassays against the Escherichia coli lysate of the recombinant clone reveal that 40% of mite allergic subjects recognize the mite glutathione S-transferase allergen. Of these sera, greater that 50% have reactivity with the recombinant mite glutathione S-transferase that is greater than 10 times the result observed with a normal control. Immunoblotting studies with sera from patients that recognize the recombinant protein reveal IgE binding to a 26-kDa protein on immunoblots of reduced mite protein extracts. The 26-kDa IgE-binding band observed on immunoblots of reduced mite proteins, corresponding to the cloned protein, is a separate and unique allergen from the 25-kDa Der p I as the apparent electrophoretic molecular mass of Der p I shifts from 25 to 30 kDa under conditions of reduction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens / analysis*
  • Allergens / chemistry
  • Animals
  • Antigens, Dermatophagoides
  • Binding Sites, Antibody
  • Blotting, Western
  • Dust / adverse effects
  • Glutathione Transferase / chemistry
  • Glutathione Transferase / immunology*
  • Glycoproteins / analysis*
  • Glycoproteins / chemistry
  • Humans
  • Immunoglobulin E / chemistry
  • Mites / enzymology
  • Mites / immunology*
  • Molecular Weight
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / immunology


  • Allergens
  • Antigens, Dermatophagoides
  • Dust
  • Glycoproteins
  • Recombinant Proteins
  • Immunoglobulin E
  • Glutathione Transferase