Abstract
Aldophosphamide (NSC 254), a putative key metabolite of cyclophosphamide, has now been isolated as a cyanohydrin derivative from an incubation mixture of cyclophosphamide with mouse liver microsomes in vitro and from the plasma of a cyclophosphamide-treated patient. The cyanohydrin has been shown to be identical with an authenic synthetic sample by mass spectrometry and combined gas chromatography-mass spectrometry.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aldehydes / isolation & purification
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Animals
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Chromatography, Gas
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Cyclophosphamide / blood
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Cyclophosphamide / metabolism*
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Humans
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In Vitro Techniques
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Mass Spectrometry
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Mice
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Microsomes, Liver / metabolism
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Nitrogen Mustard Compounds / isolation & purification*
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Organophosphorus Compounds / isolation & purification
Substances
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Aldehydes
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Nitrogen Mustard Compounds
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Organophosphorus Compounds
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Cyclophosphamide