Identification of aldophosphamide as a metabolite of cyclophosphamide in vitro and in vivo in humans

Cancer Res. 1977 Aug;37(8 Pt 1):2538-43.

Abstract

Aldophosphamide (NSC 254), a putative key metabolite of cyclophosphamide, has now been isolated as a cyanohydrin derivative from an incubation mixture of cyclophosphamide with mouse liver microsomes in vitro and from the plasma of a cyclophosphamide-treated patient. The cyanohydrin has been shown to be identical with an authenic synthetic sample by mass spectrometry and combined gas chromatography-mass spectrometry.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aldehydes / isolation & purification
  • Animals
  • Chromatography, Gas
  • Cyclophosphamide / blood
  • Cyclophosphamide / metabolism*
  • Humans
  • In Vitro Techniques
  • Mass Spectrometry
  • Mice
  • Microsomes, Liver / metabolism
  • Nitrogen Mustard Compounds / isolation & purification*
  • Organophosphorus Compounds / isolation & purification

Substances

  • Aldehydes
  • Nitrogen Mustard Compounds
  • Organophosphorus Compounds
  • Cyclophosphamide