Characterization of cDNA encoding for phosphoglucose isomerase of rice (Oryza sativa L.)

DNA Seq. 1996;6(3):127-35. doi: 10.3109/10425179609010200.

Abstract

Two types of genes (Pgi-a and Pgi-b) encoding phosphoglucose isomerase (PGI; EC 5.3.1.9) were cloned from cDNA libraries of rice cultured cells (Oryza sativa L.). Pgi-a and Pgi-b consisted of 2132 and 2030 nucleotides, respectively. The homology between these genes was 93.0% at nucleotide level. The homology scores between these genes in protein coding region and 3' non-coding region were 95.6% and 79.4%, respectively. PGI proteins encoded by Pgi-a and Pgi-b consisted of 567 and 568 amino acid, respectively, sharing 95.8% homology at amino acid sequences. Of 11 PGI genes from other plant species and organisms whose amino acid sequences had been determined, a dicotyledonous plant Clarkia lewisii PGI showed the highest homology (about 80%) with rice PGIs. GC contents at the third position of rice PGI genes were about 40%. In order to confirm the enzyme activity of the protein encoded by the rice cDNA, Pgi-a was subcloned into an expression vector, pBluescript II SKp, which was introduced into Escherichia coli. The transformant had an additional PGI activity from Pgi-a.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / genetics*
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / genetics
  • Evolution, Molecular
  • Gene Expression
  • Genes, Plant*
  • Glucose-6-Phosphate Isomerase / chemistry
  • Glucose-6-Phosphate Isomerase / genetics*
  • Glucose-6-Phosphate Isomerase / metabolism
  • Isopropyl Thiogalactoside / pharmacology
  • Molecular Sequence Data
  • Oryza / enzymology
  • Oryza / genetics*
  • Phylogeny
  • Poly A / genetics
  • Sequence Homology, Amino Acid

Substances

  • DNA, Complementary
  • Poly A
  • Isopropyl Thiogalactoside
  • Glucose-6-Phosphate Isomerase

Associated data

  • GENBANK/D45217
  • GENBANK/D45218