Directly repeated insertion of 9-nucleotide sequence detected in penicillin-binding protein 2B gene of penicillin-resistant Streptococcus pneumoniae

Antimicrob Agents Chemother. 1996 May;40(5):1257-9. doi: 10.1128/AAC.40.5.1257.

Abstract

We investigated the molecular mechanism of 50 penicillin-resistant Streptococcus pneumoniae strains (penicillin: MIC, > or = 0.125 microgram/ml) having neither class A nor class B mutations in the penicillin-binding protein 2B gene (pbp2b). An analysis of the nucleotide sequences of the pbp2b genes from seven strains revealed an unique direct repeat of 9 nucleotides (TGGTATACT) between active-site serine (residue 385) and Ser-X-Asn (residues 442 to 444) motifs. The same insertion was detected in 13 strains.

MeSH terms

  • Amino Acid Sequence
  • Aminoacyltransferases*
  • Bacterial Proteins*
  • Base Sequence
  • Carrier Proteins / genetics*
  • DNA Transposable Elements
  • DNA, Bacterial / analysis*
  • DNA, Bacterial / isolation & purification
  • Genes, Bacterial / genetics*
  • Hexosyltransferases*
  • Molecular Sequence Data
  • Muramoylpentapeptide Carboxypeptidase / genetics*
  • Mutation
  • Nucleic Acid Hybridization
  • Penicillin Resistance / genetics*
  • Penicillin-Binding Proteins
  • Peptidyl Transferases*
  • Streptococcus pneumoniae / drug effects
  • Streptococcus pneumoniae / genetics*

Substances

  • Bacterial Proteins
  • Carrier Proteins
  • DNA Transposable Elements
  • DNA, Bacterial
  • Penicillin-Binding Proteins
  • Aminoacyltransferases
  • penicillin-binding protein 2b, Streptococcus
  • Peptidyl Transferases
  • Hexosyltransferases
  • Muramoylpentapeptide Carboxypeptidase

Associated data

  • GENBANK/D42074
  • GENBANK/D42075
  • GENBANK/D82869
  • GENBANK/D82870
  • GENBANK/D82871
  • GENBANK/D82872
  • GENBANK/D82873