Abstract
We investigated the molecular mechanism of 50 penicillin-resistant Streptococcus pneumoniae strains (penicillin: MIC, > or = 0.125 microgram/ml) having neither class A nor class B mutations in the penicillin-binding protein 2B gene (pbp2b). An analysis of the nucleotide sequences of the pbp2b genes from seven strains revealed an unique direct repeat of 9 nucleotides (TGGTATACT) between active-site serine (residue 385) and Ser-X-Asn (residues 442 to 444) motifs. The same insertion was detected in 13 strains.
MeSH terms
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Amino Acid Sequence
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Aminoacyltransferases*
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Bacterial Proteins*
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Base Sequence
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Carrier Proteins / genetics*
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DNA Transposable Elements
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DNA, Bacterial / analysis*
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DNA, Bacterial / isolation & purification
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Genes, Bacterial / genetics*
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Hexosyltransferases*
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Molecular Sequence Data
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Muramoylpentapeptide Carboxypeptidase / genetics*
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Mutation
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Nucleic Acid Hybridization
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Penicillin Resistance / genetics*
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Penicillin-Binding Proteins
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Peptidyl Transferases*
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Streptococcus pneumoniae / drug effects
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Streptococcus pneumoniae / genetics*
Substances
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Bacterial Proteins
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Carrier Proteins
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DNA Transposable Elements
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DNA, Bacterial
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Penicillin-Binding Proteins
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Aminoacyltransferases
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penicillin-binding protein 2b, Streptococcus
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Peptidyl Transferases
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Hexosyltransferases
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Muramoylpentapeptide Carboxypeptidase
Associated data
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GENBANK/D42074
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GENBANK/D42075
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GENBANK/D82869
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GENBANK/D82870
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GENBANK/D82871
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GENBANK/D82872
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GENBANK/D82873