The intratracheal administration of endotoxin: X. Dexamethasone downregulates neutrophil emigration and cytokine expression in vivo

Inflammation. 1996 Apr;20(2):165-75. doi: 10.1007/BF01487403.

Abstract

Intratracheal instillation of endotoxin (LPS) causes acute pulmonary inflammation characterized by the accumulation of plasma proteins and leukocytes within the pulmonary airways. The synthetic glucocorticoid dexamethasone 1) inhibits the LPS-initiated vascular leak of plasma proteins into the airspace, 2) inhibits the LPS-initiated emigration of neutrophils and lymphocytes into the airspace in a dose-dependent fashion, and 3) inhibits LPS-initiated mRNA and/or bronchoalveolar lavage protein expression of cytokines (TNF, IL-1 and IL-6) and chemokines (MIP-1 alpha, MIP-2 and MCP-1). In conclusion, dexamethasone inhibits both the vascular and cellular aspects of acute inflammation by downregulation of a broad spectrum of inflammatory cytokines and chemokines.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Anti-Inflammatory Agents / therapeutic use
  • Bacterial Toxins / administration & dosage
  • Bacterial Toxins / toxicity*
  • Base Sequence
  • Bronchoalveolar Lavage Fluid / chemistry
  • Bronchoalveolar Lavage Fluid / cytology
  • Cells, Cultured
  • Chemotaxis, Leukocyte / drug effects*
  • Cytokines / biosynthesis*
  • Cytokines / genetics
  • Dexamethasone / pharmacology*
  • Dexamethasone / therapeutic use
  • Endotoxins / administration & dosage
  • Endotoxins / toxicity*
  • Gene Expression Regulation / drug effects*
  • Instillation, Drug
  • Leukocyte Elastase / analysis
  • Lipopolysaccharides / administration & dosage
  • Lipopolysaccharides / toxicity*
  • Male
  • Molecular Sequence Data
  • Neutrophils / drug effects*
  • Neutrophils / metabolism
  • Polymerase Chain Reaction
  • Rats
  • Rats, Inbred Lew
  • Respiratory Distress Syndrome / chemically induced*
  • Respiratory Distress Syndrome / drug therapy
  • Respiratory Distress Syndrome / pathology
  • Trachea

Substances

  • Anti-Inflammatory Agents
  • Bacterial Toxins
  • Cytokines
  • Endotoxins
  • Lipopolysaccharides
  • salmonella toxin
  • Dexamethasone
  • Leukocyte Elastase