Abstract
Neurotransmitter receptors are known to have direct roles in the modulation of neuronal morphogenesis. Previous work showed that clonal mesencephalic MN9D cells individually transfected with D2-like dopamine receptors show increased neurite outgrowth following long-term exposure to the D2-like receptor agonist quinpirole. In the current study, brief stimulation of D3 receptor-expressing cells also elicited increased neurite outgrowth, which could be mimicked by the Gi/G(o) protein activator mastoparan. Pretreatment with the Gi/G(o) protein inhibitor pertussis-toxin blocked the quinpirole- and mastoparan-mediated increases in outgrowth. These results suggest that dopamine D3 receptor stimulation has an immediate, G-protein-mediated role in neuronal morphogenesis.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cell Line
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Dopamine Agonists / pharmacology
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GTP-Binding Proteins / metabolism
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Humans
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Intercellular Signaling Peptides and Proteins
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Neurites / drug effects
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Neurites / ultrastructure*
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Peptides
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Pertussis Toxin*
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Quinpirole / antagonists & inhibitors
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Quinpirole / pharmacology
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Rats
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Receptors, Dopamine D2 / drug effects*
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Receptors, Dopamine D3
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Signal Transduction / drug effects
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Virulence Factors, Bordetella / pharmacology*
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Wasp Venoms / antagonists & inhibitors
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Wasp Venoms / pharmacology
Substances
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DRD3 protein, human
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Dopamine Agonists
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Drd3 protein, rat
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Intercellular Signaling Peptides and Proteins
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Peptides
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Receptors, Dopamine D2
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Receptors, Dopamine D3
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Virulence Factors, Bordetella
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Wasp Venoms
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Quinpirole
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mastoparan
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Pertussis Toxin
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GTP-Binding Proteins