PTX-sensitive regulation of neurite outgrowth by the dopamine D3 receptor

Neuroreport. 1996 Jan 31;7(2):573-6. doi: 10.1097/00001756-199601310-00047.


Neurotransmitter receptors are known to have direct roles in the modulation of neuronal morphogenesis. Previous work showed that clonal mesencephalic MN9D cells individually transfected with D2-like dopamine receptors show increased neurite outgrowth following long-term exposure to the D2-like receptor agonist quinpirole. In the current study, brief stimulation of D3 receptor-expressing cells also elicited increased neurite outgrowth, which could be mimicked by the Gi/G(o) protein activator mastoparan. Pretreatment with the Gi/G(o) protein inhibitor pertussis-toxin blocked the quinpirole- and mastoparan-mediated increases in outgrowth. These results suggest that dopamine D3 receptor stimulation has an immediate, G-protein-mediated role in neuronal morphogenesis.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Dopamine Agonists / pharmacology
  • GTP-Binding Proteins / metabolism
  • Humans
  • Intercellular Signaling Peptides and Proteins
  • Neurites / drug effects
  • Neurites / ultrastructure*
  • Peptides
  • Pertussis Toxin*
  • Quinpirole / antagonists & inhibitors
  • Quinpirole / pharmacology
  • Rats
  • Receptors, Dopamine D2 / drug effects*
  • Receptors, Dopamine D3
  • Signal Transduction / drug effects
  • Virulence Factors, Bordetella / pharmacology*
  • Wasp Venoms / antagonists & inhibitors
  • Wasp Venoms / pharmacology


  • DRD3 protein, human
  • Dopamine Agonists
  • Drd3 protein, rat
  • Intercellular Signaling Peptides and Proteins
  • Peptides
  • Receptors, Dopamine D2
  • Receptors, Dopamine D3
  • Virulence Factors, Bordetella
  • Wasp Venoms
  • Quinpirole
  • mastoparan
  • Pertussis Toxin
  • GTP-Binding Proteins