Glycine receptors in the rod pathway of the macaque monkey retina

Vis Neurosci. Jan-Feb 1996;13(1):101-15. doi: 10.1017/s0952523800007161.

Abstract

The distribution of glycinergic synapses in macaque monkey retina was investigated. The monoclonal antibody (mAb2b) against the alpha 1 subunit of the glycine receptor produced a punctate immunoreactivity that was localized to synapses. In central retina about 70% of the alpha 1 subunit-containing synapses were located in strata 1 and 2 of the inner plexiform layer, about 30% were located in strata 3 and 4, and immunoreactivity was absent in stratum 5. Electron microscopy showed that the majority of the synapses in strata 1 and 2 were on cone bipolar axons. The presynaptic profile always belonged to an amacrine cell. Presynaptic and postsynaptic profiles were further characterized using double-label immunofluorescence with cell-type specific antibodies against calcium-binding proteins. An antiserum against calretinin was used to label AII amacrine cells and an antiserum against recoverin was used to label flat midget bipolar cells. In the outer part of the IPL, 75% of the alpha 1-immunoreactive puncta were colocalized with calretinin-immunoreactive AII processes and 61% of the alpha 1-immunoreactive puncta were colocalized with recoverin-positive midget bipolar axons. These results suggest that the alpha 1 subunit of the glycine receptor is present at the chemical synapse made by AII amacrine cells with flat midget bipolar cells, thus providing a pathway for rod signals to reach midget ganglion cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calbindin 2
  • Calbindins
  • Eye Proteins / analysis
  • Female
  • Immunohistochemistry
  • Macaca fascicularis
  • Male
  • Microscopy, Electron
  • Nerve Tissue Proteins / analysis
  • Neuropeptides / analysis
  • Parvalbumins / analysis
  • Peptide Fragments / analysis
  • Receptors, Glycine / physiology*
  • Retinal Rod Photoreceptor Cells / physiology*
  • S100 Calcium Binding Protein G / analysis
  • Synapses / physiology

Substances

  • Calbindin 2
  • Calbindins
  • Eye Proteins
  • Nerve Tissue Proteins
  • Neuropeptides
  • Parvalbumins
  • Peptide Fragments
  • Receptors, Glycine
  • S100 Calcium Binding Protein G