Human herpesvirus 6 induces IL-8 gene expression in human hepatoma cell line, Hep G2

J Med Virol. 1996 May;49(1):34-40. doi: 10.1002/(SICI)1096-9071(199605)49:1<34::AID-JMV6>3.0.CO;2-L.


The infectivity of human herpesvirus 6 (HHV-6) in a human hepatoma cell line, Hep G2 cells, and the effect of HHV-6 on production of inflammatory cytokines in these cells were examined to analyze pathogenesis of HHV-6 in the liver. We demonstrated that Hep G2 cells were susceptible to infection with HHV-6, and produced infectious virus. Moreover, infection of Hep G2 cells by HHV-6 induced the expression of IL-8 mRNA, but not IL-1 beta. The effect on induction of IL-8 gene expression was observed only in Hep G2 cells infected with infectious virus, whereas both heat-inactivated HHV-6 and UV-irradiated HHV-6 did not change the IL-8 mRNA level in these cells. These data suggest that HHV-6 may induce the cytokine-mediated inflammatory response by infecting liver cells, which could result in liver dysfunction in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Viral / analysis
  • Base Sequence
  • Carcinoma, Hepatocellular
  • DNA Primers
  • Gene Expression Regulation
  • Heating
  • Herpesvirus 6, Human / isolation & purification
  • Herpesvirus 6, Human / physiology*
  • Humans
  • Interleukin-1 / genetics
  • Interleukin-8 / genetics*
  • Molecular Sequence Data
  • RNA, Messenger
  • Tumor Cells, Cultured
  • Ultraviolet Rays
  • Up-Regulation
  • Virus Replication


  • Antigens, Viral
  • DNA Primers
  • Interleukin-1
  • Interleukin-8
  • RNA, Messenger