Purification of Recombinant Chlamydia Trachomatis Histone H1-like Protein Hc2, and Comparative Functional Analysis of Hc2 and Hc1

Mol Microbiol. 1996 Apr;20(2):295-311. doi: 10.1111/j.1365-2958.1996.tb02618.x.

Abstract

The metabolically inactive developmental form of Chlamydia trachomatis, the elementary body, contains two very basic DNA-binding proteins with homology to eukaryotic histone H1. One of these, Hc1, is relatively well characterized and induces DNA condensation in vitro, whereas the other, Hc2, is functionally virtually uncharacterized. In this study we describe the purification of Hc2, and a detailed comparative functional analysis of Hc2 and Hc1 is presented. By gel shift assays and electron microscopy, marked differences in the nucleic acid-binding properties of Hc2 and Hc1 were observed. Furthermore, Hc2 was found to strongly inhibit translation and transcription in vitro. Our results imply that DNA condensation is not the only function of Hc2.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Bacterial / isolation & purification
  • Bacterial Proteins / genetics
  • Bacterial Proteins / isolation & purification
  • Bacterial Proteins / metabolism*
  • Base Sequence
  • Chlamydia trachomatis / metabolism*
  • DNA, Bacterial / metabolism
  • DNA, Bacterial / ultrastructure
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / isolation & purification
  • DNA-Binding Proteins / metabolism*
  • DNA-Binding Proteins / ultrastructure
  • Endopeptidases / metabolism
  • Escherichia coli
  • Factor Xa
  • Histones / genetics
  • Histones / isolation & purification
  • Histones / metabolism*
  • Histones / ultrastructure
  • Molecular Sequence Data
  • Protein Biosynthesis
  • Protozoan Proteins*
  • RNA, Viral / metabolism
  • RNA-Binding Proteins / metabolism*
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / isolation & purification
  • Recombinant Fusion Proteins / metabolism
  • Repressor Proteins / genetics
  • Repressor Proteins / isolation & purification
  • Repressor Proteins / metabolism
  • Transcription, Genetic

Substances

  • Antibodies, Bacterial
  • Bacterial Proteins
  • DNA, Bacterial
  • DNA-Binding Proteins
  • Hc1 protein, Chlamydia trachomatis
  • HctB protein, Chlamydia trachomatis
  • Histones
  • Protozoan Proteins
  • RNA, Viral
  • RNA-Binding Proteins
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • Endopeptidases
  • Factor Xa