Abstract
The effect of captopril and of its copper complex on several superoxide-dependent reactions used to detect and assay superoxide dismutase activity was studied, including pyrogallol and hematoxylin autoxidation and Nitro Blue Tetrazolium reduction. In none of these systems were superoxide dismutase-like properties of captopril/Cu apparent. Captopril/Cu decreased the yield of DMPO-OH adducts generated by KO2 but this effect may be due to the acceleration of the decay of the adduct by captopril/Cu.
MeSH terms
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Antioxidants / chemistry
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Antioxidants / pharmacology*
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Artifacts
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Captopril / chemistry
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Captopril / pharmacology*
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Copper / chemistry*
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Cyclic N-Oxides
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Free Radical Scavengers / chemistry
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Free Radical Scavengers / pharmacology*
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Nitroblue Tetrazolium / metabolism
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Spin Labels
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Spin Trapping / methods*
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Superoxide Dismutase / metabolism*
Substances
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Antioxidants
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Cyclic N-Oxides
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Free Radical Scavengers
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Spin Labels
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Nitroblue Tetrazolium
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5,5-dimethyl-1-pyrroline-1-oxide
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Copper
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Captopril
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Superoxide Dismutase