Two-dimensional electrophoretic patterns of acute-phase human serum proteins in the course of bacterial and viral diseases

Electrophoresis. 1996 Mar;17(3):612-6. doi: 10.1002/elps.1150170333.


Acute-phase serum proteins were analyzed by two-dimensional electrophoresis with isoelectric focusing in 3-10 immobilized pH gradients. Most spots were identified by reference to the plasma map in the SWISS-2DPAGE database. Serum amyloid A protein spots were identified by immunoblotting with specific antiserum and by matching determined with predicted values of electrophoretic parameters. Changes in the concentrations of alpha 1-antitrypsin, leucine-rich glycoprotein, haptoglobin, serum retinol-binding protein and transthyretin were quantitated by densitometry of silver-stained gels. Electrophoretic patterns from 18 patients with bacterial diseases and 16 patients with viral diseases were compared. The incidence of serum amyloid A protein spots was 18/18 in bacterial diseases and 6/16 in viral diseases. As the the other reactants studied, variations were simultaneous in bacterial disease and tended to be staggered in viral diseases.

MeSH terms

  • Acute-Phase Proteins / analysis*
  • Bacterial Infections / blood*
  • Chickenpox / blood
  • Chickenpox / metabolism
  • Child
  • Child, Preschool
  • Electrophoresis, Gel, Two-Dimensional*
  • Haemophilus Infections / blood
  • Haemophilus Infections / metabolism
  • Haemophilus influenzae / isolation & purification
  • Humans
  • Measles / blood
  • Measles / metabolism
  • Mumps / blood
  • Mumps / metabolism
  • Salmonella / isolation & purification
  • Salmonella Infections / blood
  • Salmonella Infections / metabolism
  • Streptococcal Infections / blood
  • Streptococcal Infections / metabolism
  • Streptococcus pyogenes / isolation & purification
  • Virus Diseases / blood*


  • Acute-Phase Proteins