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Comparative Study
. 1996 Feb;17(1):69-77.
doi: 10.1007/BF00140325.

Cloning of the cDNA Encoding Rat Myosin Heavy chain-A and Evidence for the Absence of Myosin Heavy chain-B in Cultured Rat Mast (RBL-2H3) Cells

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Comparative Study

Cloning of the cDNA Encoding Rat Myosin Heavy chain-A and Evidence for the Absence of Myosin Heavy chain-B in Cultured Rat Mast (RBL-2H3) Cells

O H Choi et al. J Muscle Res Cell Motil. .

Abstract

The complete amino acid sequence (1961 amino acids) of a vertebrate cellular myosin heavy chain-A was deduced from cDNA clones of a secretory rat mast cell line, the RBL-2H3 cell. The rat, human and chicken cellular myosin heavy chain-A exhibited high similarity in domains that allow binding of ATP and actin. The amino acid sequence of non-muscle myosin heavy chain-A from rat was 96% identical to that in human and 92% identical to that in chicken. Northern blot analysis of mRNA indicated the presence of single message of 7.4 kilobases. Northern blot, reverse-transcriptase polymerase chain reaction, and Western blot with isoform-specific antibodies indicated that RBL-2H3 cells expressed exclusively myosin heavy chain-A. Unlike rat PC12 cells, as well as a wide variety of other cultured cells and tissues, myosin heavy chain-B mRNA and protein were not detectable in RBL-2H3 cells. Because RBL-2H3 cells can be stimulated to release secretory granules as well as newly generated arachidonic acid and cytokines but lack myosin heavy chain-B, this cell line may provide a unique model to study the role of myosin heavy chain-A in cellular responses to antigen and other stimulants.

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References

    1. Proc Natl Acad Sci U S A. 1990 Feb;87(3):1164-8 - PubMed
    1. J Biol Chem. 1993 Jan 25;268(3):1749-56 - PubMed
    1. Prog Allergy. 1988;42:123-84 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Oct;86(20):7726-30 - PubMed
    1. Nature. 1989 Oct 12;341(6242):549-51 - PubMed

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