Quantification of osteopontin in human plasma with an ELISA: basal levels in pre- and postmenopausal women

Clin Biochem. 1996 Jun;29(3):231-9. doi: 10.1016/0009-9120(96)84728-a.

Abstract

Objectives: To develop an immunoassay for osteopontin (OPN), a secreted phosphoglycoprotein that is implicated in a number of human diseases, and establish basal plasma OPN levels in healthy women.

Design and methods: An antigen-capture ELISA was developed to quantity OPN in plasma using a combination of mouse monoclonal and rabbit polyclonal antibodies. Basal OPN levels were determined in blood plasma of 21 pre- and 14 postmenopausal women obtained at 7-day intervals over a 4-week period.

Results: A group of 35 healthy women had a median OPN level of 31 micrograms/L (range = 14-64 micrograms/L). Comparison between pre- and postmenopausal women showed that their 4-week average OPN levels did not differ significantly (p > 0.16, Mann-Whitney test), and that levels in each premenopausal individual remained constant during the menstrual cycle, unaffected by cyclical levels of leuteinizing hormone and progesterone.

Conclusion: Systematic quantification of plasma OPN can now be done by ELISA, which was used to establish basal plasma OPN levels in a group of healthy women. Levels in pre- and postmenopausal women appeared relatively stable over a 4-week period.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Blotting, Western
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Female
  • Fibrocystic Breast Disease / blood
  • Humans
  • Mastitis / blood
  • Menstrual Cycle / blood
  • Mice
  • Middle Aged
  • Osteopontin
  • Postmenopause / blood*
  • Rabbits
  • Sialoglycoproteins / blood*
  • Sialoglycoproteins / physiology*

Substances

  • SPP1 protein, human
  • Sialoglycoproteins
  • Spp1 protein, mouse
  • Osteopontin