Diversity of intrathecal antibody synthesis against HTLV-I and its relation to HTLV-I associated myelopathy

J Neurol. 1996 May;243(5):393-400. doi: 10.1007/BF00868998.


The humoral immune response against human T-cell lymphotropic virus type I (HTLV-I) in the central nervous system (CNS) compartment and in the blood was investigated by enzyme immunoassay using 16 synthetic peptides corresponding to HTLV-I core and envelope sequences. We evaluated paired samples of cerebrospinal fluid and serum from HTLV-I seropositive Japanese patients, classified as follows: HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP; n = 39), patients with spinal cord disease ascribed to either HAM/TSP or to some concomitant, HTLV-I-unrelated disease (possible HAM/TSP; n = 6) or carriers without any clinical signs of HAM/TSP (n = 15). HTLV-I-peptide-specific intrathecal antibody synthesis was found in 79% of HAM/TSP patients, but only in 20% of carriers without HAM/TSP. The group of carriers without HAM/TSP showed local synthesis for some peptides (on average 0.3 peptides per patient). In most HAM/TSP patients, however, there was a diverse intrathecal immune response to several HTLV-I synthetic peptides (on average against 3.6 peptides per HAM/TSP patient), most frequently against gag p19 100-130, env gp21 458-488, and env gp46 175-199 and 288-317. The intrathecal antibody synthesis against several HTLV-I determinants may represent a pathogenic immune response in HAM/TSP and is possibly related to the infiltration of virus-infected T-cells in the spinal cord.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Viral / blood
  • Antibodies, Viral / cerebrospinal fluid*
  • Antibody Formation
  • Antibody Specificity
  • Case-Control Studies
  • Evaluation Studies as Topic
  • Gene Products, gag / immunology
  • Human T-lymphotropic virus 1 / immunology*
  • Humans
  • Immunoenzyme Techniques
  • Immunoglobulin G / blood
  • Immunoglobulin G / cerebrospinal fluid*
  • Molecular Sequence Data
  • Paraparesis, Tropical Spastic / blood
  • Paraparesis, Tropical Spastic / cerebrospinal fluid
  • Paraparesis, Tropical Spastic / immunology*
  • Peptides / immunology*
  • Viral Envelope Proteins / immunology


  • Antibodies, Viral
  • Gene Products, gag
  • Immunoglobulin G
  • Peptides
  • Viral Envelope Proteins